Holbrook is a village situated close to the northern shore of the estuary of the River Stour, in Suffolk, England. It is located on the Shotley Peninsula in Babergh district, around 5 miles (8.0 km) south of Ipswich.
To the south of the village is the Royal Hospital School, which moved to this site in 1933 after having been housed at Greenwich Hospital since 1693.
Lower Holbrook is a hamlet between the villages of Holbrook and Harkstead.
An electoral ward of the same name exists. The population of this ward at the 2011 census was 2,467. The village has two pubs, The Compasses and The Swan, a Co-op store, a butcher, and a village hall. The area is served by a primary school and Holbrook Academy, which shares a site with the Peninsula Sports Centre. The parish church, dedicated to All Saints, is a Grade II* listed building. The village also has Methodist church. During the Battle of Britain, a German military aircraft crashed into a field near the village. Contemporary newspaper accounts identified the aircraft as a Messerschmitt.